Dynamic processes in membranes can be followed by epifluorescence microscopy using fluorescent probes and sCMOS cameras, but undesired out of focus fluorescence reduces the signal-to-noise ratio and image resolution.
Working on sub-second time scales, confocal microscopy is ideally suited to following membrane dynamics, since spatial resolution is increased by measuring only the fluorescence of focused thin sample sections, removing out-of-focus fluorescence. In 3D membrane structures, confocal microscopy allows the acquisition of consecutive scans at varying depths. These scans are then processed to obtain a 3D view of the sample.
The Zeiss Axio Imager provides a high performance upright, transmitted light microscope stand designed for constant operation with a high sample throughput in demanding applications including confocal and fluorescence imaging.
The motorised focus (z-axis) drive ensures precise focusing movements of 10nm step size on up to 9 kg heavy sample stages and over long periods of operation.
The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The Zyla 5.5 USB3 is the latest 16 bit sCMOS based camera from Andor. It offers high speed, high sensitivity imaging from a compact device that is ideal for low light microscopy.
|Thermal stabilization (°C )||0|
|Read-out noise (e- rms)||1.2|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||25000 : 1|
|Quantum efficiency||Up to 60%|
|Maximum frame rate (fps)||40|
|Read-out modes||Rolling and global shutter|
|Dimensions (mm)||133 x 82 x 80|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
|Type||Plan Apochromat SF25|
|Working Distance (mm)||0.55|
The Prior Scientific Lumen 200 PRO is a 200W metal arc lamp fluorescence illumination system designed for use with fully automated microscopy systems.
Located remote from the microscope for no vibration, the Lumen 200 PRO has an integrated six position motorised filter wheel for fast switching of the excitation wavelength and motorised light attenuation and shuttering. This allows intensity selection over 100 increments for balancing exposure with the sensitivity of your cells and fluorescent markers.
The Lumen 220 PRO model offers extended transmission in UV and IR.
Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: