Dynamic processes in membranes can be followed by epifluorescence microscopy using fluorescent probes and sCMOS cameras, but undesired out of focus fluorescence reduces the signal-to-noise ratio and image resolution.
Working on sub-second time scales, confocal microscopy is ideally suited to following membrane dynamics, since spatial resolution is increased by measuring only the fluorescence of focused thin sample sections, removing out-of-focus fluorescence. In 3D membrane structures, confocal microscopy allows the acquisition of consecutive scans at varying depths. These scans are then processed to obtain a 3D view of the sample.
The Zeiss Axio Imager provides a high performance upright, transmitted light microscope stand designed for constant operation with a high sample throughput in demanding applications including confocal and fluorescence imaging.
The motorised focus (z-axis) drive ensures precise focusing movements of 10nm step size on up to 9 kg heavy sample stages and over long periods of operation.
The Zyla 5.5 USB3 is the latest 16 bit sCMOS based camera from Andor. It offers high speed, high sensitivity imaging from a compact device that is ideal for low light microscopy.
|Thermal stabilization (°C )||0|
|Read-out noise (e- rms)||1.2|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||25000 : 1|
|Quantum efficiency||Up to 60%|
|Maximum frame rate (fps)||40|
|Read-out modes||Rolling and global shutter|
|Dimensions (mm)||133 x 82 x 80|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
|Type||Plan Apochromat SF25|
|Working Distance (mm)||0.55|
Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: