applications

Development (e.g. C.Elegans, Zebrafish and Drosophila)

Confocal microscopy is an invaluable tool in developmental biology leading to improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues.

The following is a suggested Clarity system configuration for this application:

The Olympus BX is a fully motorised upright microscope which can be used in Fluorescence, Differential Interference Contrast (DIC), Phase Contrast, Simple Polarised Light, Brightfield and Darkfield observation modes.

The BX range is unusually focused by driving the motorised nosepiece in the z-direction. This means that the microscope stage remains fixed and affords greater stability.

The BX stage can be driven using its ultrasonic Piezo motor technology, which provide silent, smooth and precise operation, or it can be positioned manually; allowing for fast gross sample alignment. In motorised or manual operation the BX's high accuracy encoders provide continuous read-out of the X and Y position of the stage.

Key features:

  • Fully motorised
  • Motorised focus using the nosepiece z-direction
  • Motorised objective change
  • Touch panel display / control
  • Computer controllable

aurox confocal microscope Olympus BX - upright

The Zyla 5.5 USB3 is the latest 16 bit sCMOS based camera from Andor. It offers high speed, high sensitivity imaging from a compact device that is ideal for low light microscopy.

Key specifications:
Thermal stabilization (°C )0
Read-out noise (e- rms)1.2
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range25000 : 1
Quantum efficiencyUp to 60%
Maximum frame rate (fps)40
Read-out modesRolling and global shutter
Lightsheet modeYes
InterfaceUSB 3.0
SensorsCMOS
Dimensions (mm)133 x 82 x 80

aurox confocal microscope Andor Zyla 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates