applications

Development (e.g. C.Elegans, Zebrafish and Drosophila)

Confocal microscopy is an invaluable tool in developmental biology leading to improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues.

The following is a suggested Clarity system configuration for this application:

The Nikon SMZ25 is a high resolution research stereomicroscope combining 25:1 zoom and high NA to provide exceptionally bright, uniform fluorescence.

The SMZ25 provides macro and micro imaging for convenient viewing and manipulation of individual cells to gross specimens.

Combining innovative optics and a fly-eye lens, the SMZ25 provides crystal clear fluorescent images of improved signal-to-noise, even for low-excitation light applications including cell division, whilst at low magnifications, the 35 mm field-of-view allows imaging of a whole 35 mm dish with uniform brightness.

Key features:

  • 25:1 zoom (0.63x 15.75x)
  • Macro and micro imaging in one microscope
  • Automatic zoom factor adjust for same F.O.V on objective switch
  • Fly-eye lens uniformly illuminates entire F.O.V
  • High S/N with increased transmission even at low magnifications
  • On-axis imaging for digital images
  • Motorised zoom and focus
  • Software controllable

aurox confocal microscope Nikon SMZ25 - zoom

The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.

Features:

  • Laser free confocal
  • Full spectrum (370 - 700 nm)
  • Excellent confocality
  • Unique single camera design with large field of view
  • High throughput
  • Highly cost effective
  • A range of interchangeable filter turrets
  • Real-time control and viewing (up to 50 fps)
  • Suitable for fixed and live specimens


Key Specifications
Operating principleStructured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample
Confocality (PSF) 3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens
Widefield By-passSelectable mode completely removes disk from the imaging path
Disk rotation speed3000 rpm
Minimum exposure20 ms (1 full disk rotation)
Frame rates (max)22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning
Spectral channels4 user-replaceable cubes on a fast internal filter turret
Filter switching time<200 ms
Excitation range370 - 700 nm
Emission range410 - 750 nm



aurox confocal microscope Aurox Clarity LFC

The Zyla 5.5 USB3 is the latest 16 bit sCMOS based camera from Andor. It offers high speed, high sensitivity imaging from a compact device that is ideal for low light microscopy.

Key specifications:
Thermal stabilization (°C )0
Read-out noise (e- rms)1.2
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range25000 : 1
Quantum efficiencyUp to 60%
Maximum frame rate (fps)40
Read-out modesRolling and global shutter
Lightsheet modeYes
InterfaceUSB 3.0
SensorsCMOS
Dimensions (mm)133 x 82 x 80

aurox confocal microscope Andor Zyla 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.

Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.

Features:

  • Search, tag, and manage your data
  • Organize and manage full experiments
  • Use premier volume rendering in 3D/4D
  • Detect objects using Spots and Surfaces
  • Interactive intuitive workflow
  • Process and visualise data acquired from almost any microscope.
  • Customisable with a wide range of additional, optional modules for specialist applications

aurox confocal microscope Bitplane Imaris

The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.

With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.

This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.

Features:

  • High end Windows 10 Shuttle workstation
  • Acquisition bandwidth for USB3 sCMOS camera support
  • SSD supports real time data acquisition
  • Multi-core processors for multi-threaded software
  • High performance graphics card with OpenGL
  • Includes 24" LCD Monitor
  • Real-time volume rendering

aurox confocal microscope Windows 10 Shuttle PC workstation

The Cool LED pE-4000 is a broad spectrum (365-770 nm) illumination system for fluorescence microscopy with 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope.

For consistency and ease of use the system can be operated as a ‘White’ illumination system using simple on/off and intensity buttons, or in “Advanced” mode for total control of the excitation source for improved imaging and for selection and control of individual wavelength settings.

Features:

  • Broad LED spectrum: 365 – 770 nm
  • Four channel wavelength-grouping design
  • Individual control of selected LED wavelengths
  • Instant on/off - no shutters, no warm up or cool down
  • Precise intensity control in 1% steps (0-100%)
  • Excellent uniformity over field of view
  • Lamp life expected >25000 hours of operating time

aurox confocal microscope Cool LED pE-4000

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates