Spinning disk confocal microscopy systems are ideally suited to observing fast biological events occurring within small volumes.

For example, in the study of blood vessel disease, intracellular Ca2+ can be observed signalling between the projections of endothelial cells and the adjacent smooth muscle cells in the blood vessel wall.

aurox confocal microscope Endothelial cells

The following is a suggested Clarity system configuration for this application:

The Nikon Eclipse FN-1 is a fixed stage upright microscope which has been specially designed for electrophysiological research applications. The FN-1 allows you to go deeper into your specimen due to its water dipping objective with depth-induced aberration correction.

The FN-1 delivers longer working distances, slimmer profiles, better approach angles and aberration free images.

Key features:

  • Removable condenser, sub-stage and turret for greater access and space
  • ON/OFF mechanism (filter turret, changers for the optical path, magnification and condenser filter) noise reduction
  • 15 mm retraction for objective magnification switching

aurox confocal microscope Nikon Eclipse FN-1 - upright

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

aurox confocal microscope PCO Edge 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

TypePlan Apochromat
Numerical Aperture0.95
Working Distance (mm)0.25 – 0.16

aurox confocal microscope Nikon CFI Plan Apo Lambda 40x

Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates