In live cell chemotaxis studies the degree and direction of movement of a motile cell or organism is studied as a response to the changing concentration of a chemical substance.
Real-time chemotaxis may be studied using time-lapse confocal microscopy.
A suitable confocal microscope system includes a stage top incubator with heating plate, micromanipulation equipment, fluorescent light source and appropriate filters.
The Leica DMi8 is an inverted, transmitted light microscope stand for Brightfield, Integrated Modulation Contrast (IMC), Phase Contrast, Darkfield, Differential Interference Contrast (DIC) and Fluorescence applications.
The fully motorised Leica DMi8 offers high precision 3D image reconstruction of large specimens through a combination of its closed loop focus with 20nm positional accuracy and extended 12mm travel range.
A modular system for ease of upgrade and support, the DMi8 features an easy to use touch panel control which can be programmed with your personal preferences. The DMi8 makes tasks convenient and reproducible by automatically adjusting settings.
Illumination, parfocality, brightness and diaphragm position are automatically adjusted based on the selected contrast method, whilst the Adaptive Focus Control (AFC) constantly adjusts and maintains focus.
The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.
|Thermal stabilization (°C )||0 / +5|
|Read-out noise (e- med)||1.0|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||30000 : 1|
|Exposure time (secs)||0.1 to 2|
|Maximum frame rate (fps)||30|
|Read-out modes||Rolling or global / global reset functionality|
|Sensor||Black and white sCMOS|
|Dimensions (mm)||102 x 79 x 70|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.
Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
|Working Distance (mm)||0.14|
The Prior Scientific Lumen 200 PRO is a 200W metal arc lamp fluorescence illumination system designed for use with fully automated microscopy systems.
Located remote from the microscope for no vibration, the Lumen 200 PRO has an integrated six position motorised filter wheel for fast switching of the excitation wavelength and motorised light attenuation and shuttering. This allows intensity selection over 100 increments for balancing exposure with the sensitivity of your cells and fluorescent markers.
The Lumen 220 PRO model offers extended transmission in UV and IR.
The Prior Scientific H117 motorised XY stage series is suitable for use with most new inverted microscopes and has a completely flat top for easy access.
The H117 series includes Intelligent Scanning Technology (ITS), which allows the stage to be programmed for optimum set-up and performance. ITS also allows the ProScan™ III controller to adjust for and maintain superior orthogonality and metric accuracy.
The OkoLab stage top incubator chamber creates the ideal environment for live cell imaging on the microscope stage.
Incubators are available to fit any microscope XY stage or piezo insert and all connect to Okolab temperature, gas and humidity controllers.
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: