applications

Motility and chemotaxis assays

In live cell chemotaxis studies the degree and direction of movement of a motile cell or organism is studied as a response to the changing concentration of a chemical substance.

Real-time chemotaxis may be studied using time-lapse confocal microscopy.

A suitable confocal microscope system includes a stage top incubator with heating plate, micromanipulation equipment, fluorescent light source and appropriate filters.

aurox confocal microscope title

The following is a suggested Clarity system configuration for this application:

The Leica DMi is an inverted, transmitted light microscope stand for Brightfield, Integrated Modulation Contrast (IMC), Phase Contrast, Darkfield, Differential Interference Contrast (DIC) and Fluorescence applications.

The fully motorised Leica DMi offers high precision 3D image reconstruction of large specimens through a combination of its closed loop focus with 20nm positional accuracy and extended 12mm travel range.

A modular system for ease of upgrade and support, the DMi features an easy to use touch panel control which can be programmed with your personal preferences. The DMi makes tasks convenient and reproducible by automatically adjusting settings.

Illumination, parfocality, brightness and diaphragm position are automatically adjusted based on the selected contrast method, whilst the Adaptive Focus Control (AFC) constantly adjusts and maintains focus.

Key features:

  • Fully motorised stand
  • Adaptive Focus Control (AFC)
  • Infinity Port (additional incident illumination port)
  • Leica application Suite X software
  • Remote mobile software module
  • TFT display
  • Modular for ease of maintenance and upgrade

aurox confocal microscope Leica DMi - inverted

The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.

Features:

  • Laser free confocal
  • Full spectrum (370 - 700 nm)
  • Excellent confocality
  • Unique single camera design with large field of view
  • High throughput
  • Highly cost effective
  • A range of interchangeable filter turrets
  • Real-time control and viewing (up to 50 fps)
  • Suitable for fixed and live specimens




Key Specifications
Operating principleStructured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample
Confocality (PSF) 3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens
Widefield By-passSelectable mode completely removes disk from the imaging path
Disk rotation speed3000 rpm
Minimum exposure20 ms (1 full disk rotation)
Frame rates (max)22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning
Spectral channels4 user-replaceable cubes on a fast internal filter turret
Filter switching time<200 ms
Excitation range370 - 700 nm
Emission range410 - 750 nm

aurox confocal microscope Aurox Clarity LFC

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

aurox confocal microscope PCO Edge 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.

With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.

This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.

Features:

  • High end Windows 10 Shuttle workstation
  • Acquisition bandwidth for USB3 sCMOS camera support
  • SSD supports real time data acquisition
  • Multi-core processors for multi-threaded software
  • High performance graphics card with OpenGL
  • Includes 24" LCD Monitor
  • Real-time volume rendering

aurox confocal microscope Windows 10 Shuttle PC workstation

Specifications
Type Plan Apochromat
Magnification63x
Numerical Aperture1.40
Working Distance (mm)0.14
ImmersionOil

aurox confocal microscope Leica HC PL APO 63x/1.40 OIL CS2

The Prior Scientific Lumen 200 PRO is a 200W metal arc lamp fluorescence illumination system designed for use with fully automated microscopy systems.

Located remote from the microscope for no vibration, the Lumen 200 PRO has an integrated six position motorised filter wheel for fast switching of the excitation wavelength and motorised light attenuation and shuttering. This allows intensity selection over 100 increments for balancing exposure with the sensitivity of your cells and fluorescent markers.

The Lumen 220 PRO model offers extended transmission in UV and IR.

Features:

  • 2000 hour life, 200 Watt metal arc lamp
  • Stabilised DC power supply for consistent illumination
  • Quiet operation
  • Easy to view display screen for bulb life indication and alarm description
  • Knurled light guide clamping knob for secure light guide installation
  • Adapters available for all modern research microscopes
  • Pre-aligned bulb for easy replacement
  • Integrated motorised six position 25mm filter wheel
  • Motorised light attenuator, 0-100% in 1% increments
  • Compatible with ProScan™ III controllers

aurox confocal microscope Prior Scientific Lumen 200/220 PRO

The Prior Scientific H117 motorised XY stage series is suitable for use with most new inverted microscopes and has a completely flat top for easy access.

The H117 series includes Intelligent Scanning Technology (ITS), which allows the stage to be programmed for optimum set-up and performance. ITS also allows the ProScan™ III controller to adjust for and maintain superior orthogonality and metric accuracy.

Key features:

  • Repeatability, 1um or better
  • Flat top for easy access
  • Quiet and reliable
  • Compatible with most incubators
  • Wide range of sample holders

aurox confocal microscope Prior Scientific H117 series XY motor stages

The OkoLab stage top incubator chamber creates the ideal environment for live cell imaging on the microscope stage.

Incubators are available to fit any microscope XY stage or piezo insert and all connect to Okolab temperature, gas and humidity controllers.

Features:

  • Temperature range: 3°C above ambient to 60°C
  • Temperature accuracy: 0.1°C in sample feedback mode, 0.3°C in chamber feedback mode
  • Compatible with Bold Line T, UNO Combined and H401-T Controllers
  • Embedded temperature sensor in heated glass lid and chamber body
  • Interchangeable magnetic inserts for petri / slides / multi-well plates
  • Magnetic locks hold petri / slides / multi-well plates in position
  • Perfusion holes for inlet / outlet tubes
  • Sliding lid for easy sample access
  • Optional: lids for Koehler illumination, laser safety and injection during imaging

aurox confocal microscope OkoLab Microscope Stage Incubator

Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates