Motility and chemotaxis assays

In live cell chemotaxis studies the degree and direction of movement of a motile cell or organism is studied as a response to the changing concentration of a chemical substance.

Real-time chemotaxis may be studied using time-lapse confocal microscopy.

A suitable confocal microscope system includes a stage top incubator with heating plate, micromanipulation equipment, fluorescent light source and appropriate filters.

aurox confocal microscope title

The following is a suggested Clarity system configuration for this application:

The Leica DMi is an inverted, transmitted light microscope stand for Brightfield, Integrated Modulation Contrast (IMC), Phase Contrast, Darkfield, Differential Interference Contrast (DIC) and Fluorescence applications.

The fully motorised Leica DMi offers high precision 3D image reconstruction of large specimens through a combination of its closed loop focus with 20nm positional accuracy and extended 12mm travel range.

A modular system for ease of upgrade and support, the DMi features an easy to use touch panel control which can be programmed with your personal preferences. The DMi makes tasks convenient and reproducible by automatically adjusting settings.

Illumination, parfocality, brightness and diaphragm position are automatically adjusted based on the selected contrast method, whilst the Adaptive Focus Control (AFC) constantly adjusts and maintains focus.

Key features:

  • Fully motorised stand
  • Adaptive Focus Control (AFC)
  • Infinity Port (additional incident illumination port)
  • Leica application Suite X software
  • Remote mobile software module
  • TFT display
  • Modular for ease of maintenance and upgrade

aurox confocal microscope Leica DMi - inverted

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

aurox confocal microscope PCO Edge 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

Type Plan Apochromat
Numerical Aperture1.40
Working Distance (mm)0.14

aurox confocal microscope Leica HC PL APO 63x/1.40 OIL CS2

The Prior Scientific H117 motorised XY stage series is suitable for use with most new inverted microscopes and has a completely flat top for easy access.

The H117 series includes Intelligent Scanning Technology (ITS), which allows the stage to be programmed for optimum set-up and performance. ITS also allows the ProScan™ III controller to adjust for and maintain superior orthogonality and metric accuracy.

Key features:

  • Repeatability, 1um or better
  • Flat top for easy access
  • Quiet and reliable
  • Compatible with most incubators
  • Wide range of sample holders

aurox confocal microscope Prior Scientific H117 series XY motor stages

The OkoLab stage top incubator chamber creates the ideal environment for live cell imaging on the microscope stage.

Incubators are available to fit any microscope XY stage or piezo insert and all connect to Okolab temperature, gas and humidity controllers.


  • Temperature range: 3°C above ambient to 60°C
  • Temperature accuracy: 0.1°C in sample feedback mode, 0.3°C in chamber feedback mode
  • Compatible with Bold Line T, UNO Combined and H401-T Controllers
  • Embedded temperature sensor in heated glass lid and chamber body
  • Interchangeable magnetic inserts for petri / slides / multi-well plates
  • Magnetic locks hold petri / slides / multi-well plates in position
  • Perfusion holes for inlet / outlet tubes
  • Sliding lid for easy sample access
  • Optional: lids for Koehler illumination, laser safety and injection during imaging

aurox confocal microscope OkoLab Microscope Stage Incubator

Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates