Imaging of the cellular composition of plant tissue is necessary for our understanding of plant development but light and electron microscopy require the labour intensive production of tissue sections and often result in poorly determined 3D images of the tissue.
Confocal microscopy overcomes these problems by allowing the collection of a series of consecutive optical sections through intact fluorescently labelled plant tissue.
The Nikon Eclipse Ti-E inverted microscope has been designed for the most advanced live cell imaging applications including TIRF, confocal imaging, FRET, photo activation and microinjection.
The high speed motorised nosepiece, fluorescence filters and microscope stage allow for high throughput screening during multi-dimensional experiments, whilst the automatic focus correction system continuously corrects for any drift during long duration time-lapse imaging.
The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.
|Thermal stabilization (°C )||0 / +5|
|Read-out noise (e- med)||1.0|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||30000 : 1|
|Exposure time (secs)||0.1 to 2|
|Maximum frame rate (fps)||30|
|Read-out modes||Rolling or global / global reset functionality|
|Sensor||Black and white sCMOS|
|Dimensions (mm)||102 x 79 x 70|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.
Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
|Working Distance (mm)||0.13|
The Excilitas X-Cite 120PC Q is a computer controlled fluorescence microscope light source offering rich spectral sample excitation and a uniformly illuminated field of view from a 120W lamp which has been pre-aligned for convenience and has a guaranteed 2000 hours lifetime.
The X-cite 120PC Q also features software control and a standard adjustable iris, which may help minimise photo-bleaching.
|Illumination system includes||Lamp module, liquid light guide, microscope adaptor, software CD, grounded and shielded power cord, 9-pin serial cable, manual and quick start guide|
|Lamp||Pre-aligned proprietary 120W Mercury Vapor Short Arc|
|Lamp Life (hrs)||2000 guaranteed|
|Foot pedal operation||Yes|
|Warm-up period (s)||90 (typical)|
|Dimensions (mm)||139 x 165 x 342|
|Adapters||Compatible with wide-field fluorescence microscopes from all major manufacturers|
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: