applications

Plant tissue

Imaging of the cellular composition of plant tissue is necessary for our understanding of plant development but light and electron microscopy require the labour intensive production of tissue sections and often result in poorly determined 3D images of the tissue.

Confocal microscopy overcomes these problems by allowing the collection of a series of consecutive optical sections through intact fluorescently labelled plant tissue.

aurox confocal microscope Brassica flower bud

The following is a suggested Clarity system configuration for this application:

The Nikon Eclipse Ti-E inverted microscope has been designed for the most advanced live cell imaging applications including TIRF, confocal imaging, FRET, photo activation and microinjection.

The high speed motorised nosepiece, fluorescence filters and microscope stage allow for high throughput screening during multi-dimensional experiments, whilst the automatic focus correction system continuously corrects for any drift during long duration time-lapse imaging.

Key features:

  • Motorised focus (z-axis)
  • 25nm minimum focal step size
  • 3-ports
  • Motorised stage with encoders
  • Motorised port switching
  • Automatic focus correction
  • External phase contrast unit
  • Software controllable

aurox confocal microscope Nikon Eclipse Ti-E - inverted

The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.

Features:

  • Laser free confocal
  • Full spectrum (370 - 700 nm)
  • Excellent confocality
  • Unique single camera design with large field of view
  • High throughput
  • Highly cost effective
  • A range of interchangeable filter turrets
  • Real-time control and viewing (up to 50 fps)
  • Suitable for fixed and live specimens


Key Specifications
Operating principleStructured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample
Confocality (PSF) 3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens
Widefield By-passSelectable mode completely removes disk from the imaging path
Disk rotation speed3000 rpm
Minimum exposure20 ms (1 full disk rotation)
Frame rates (max)22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning
Spectral channels4 user-replaceable cubes on a fast internal filter turret
Filter switching time<200 ms
Excitation range370 - 700 nm
Emission range410 - 750 nm



aurox confocal microscope Aurox Clarity LFC

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

aurox confocal microscope PCO Edge 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.

Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.

Features:

  • Search, tag, and manage your data
  • Organize and manage full experiments
  • Use premier volume rendering in 3D/4D
  • Detect objects using Spots and Surfaces
  • Interactive intuitive workflow
  • Process and visualise data acquired from almost any microscope.
  • Customisable with a wide range of additional, optional modules for specialist applications

aurox confocal microscope Bitplane Imaris

The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.

With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.

This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.

Features:

  • High end Windows 10 Shuttle workstation
  • Acquisition bandwidth for USB3 sCMOS camera support
  • SSD supports real time data acquisition
  • Multi-core processors for multi-threaded software
  • High performance graphics card with OpenGL
  • Includes 24" LCD Monitor
  • Real-time volume rendering

aurox confocal microscope Windows 10 Shuttle PC workstation

Specifications
TypePlan Apochromat
Magnification60x
Numerical Aperture1.4
Working Distance (mm)0.13
ImmersionOil

aurox confocal microscope Nikon CFI Plan Apo Lambda 60x Oil

The Excilitas X-Cite 120PC Q is a computer controlled fluorescence microscope light source offering rich spectral sample excitation and a uniformly illuminated field of view from a 120W lamp which has been pre-aligned for convenience and has a guaranteed 2000 hours lifetime.

The X-cite 120PC Q also features software control and a standard adjustable iris, which may help minimise photo-bleaching.

Technical Specifications:

Illumination system includesLamp module, liquid light guide, microscope adaptor, software CD, grounded and shielded power cord, 9-pin serial cable, manual and quick start guide
LampPre-aligned proprietary 120W Mercury Vapor Short Arc
Lamp Life (hrs)2000 guaranteed
Software interfaceYes
RS-232Yes
Adjustable IrisYes
Foot pedal operationYes
Warm-up period (s)90 (typical)
Dimensions (mm)139 x 165 x 342
Weight (kg)3.18
AdaptersCompatible with wide-field fluorescence microscopes from all major manufacturers

aurox confocal microscope Excilitas X-Cite 120PC Q

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates