Introducing the Aurox Clarity, the compact, easy and affordable route to laser-free confocal imaging using your existing fluorescence microscope.
Designed to fit virtually any microscope, Clarity uses Aurox’s patented structured illumination technology and a spinning disc to achieve high resolution, high quality confocal images, fast, easily and affordably.
Clarity packs 15 years of Aurox confocal microscopy and optical technology into the World’s most compact, high performance confocal imager.
Clarity can be directly coupled to a wide range of modern large field of view sCMOS and CCD detectors, including:
Clarity incorporates an internal 4-position filter cube turret, accessed via an external door. The turret accepts Aurox’s combined emission and excitation filter cubes which are user exchangeable and quick and easy to replace without tools.A wide range of Aurox filter cubes are available to cover the commonly used bandwidths. Custom filter cubes are available for specific research applications.
Clarity can be connected directly or via a light guide to a wide range of LED or metal halide light sources. Clarity is free from laser safety restrictions and benefits from low photo-toxicity, low-bleaching, low maintenance and low cost of ownership.
Clarity uses Aurox’s patented structured illumination spinning disc technology. This is based on a grid pattern which allows up to 50% more light through than traditional (Nipkov) pin-hole designs. The results are high resolution, high quality confocal images collected fast and without the need for lasers.Each disc has 3 grid pattern sizes affording 3 sectioning modes and thereby image optimisation for resolution or speed.
Clarity has a standard microscope C-mount connector and a range of coupling adaptors / spacers to fit historical and new microscope models from all major manufacturers.
A high speed, ClarityHS version is available featuring a 10 ms minimum exposure. The ClarityHS provides up to 100 frames per second full frame video capture, making it ideally suited for higher resolution 3D time lapse imaging.ClarityHS requires an appropriate camera and computer to operate in high speed mode.
Clarity is typically delivered with an instrument control computer installed with the Aurox Visionary software. The computer is connected by USB2 and can be either a compact NUC or a Shuttle PC.
Clarity comes with the Aurox Visionary device control and image acquisition software.Fast and easy to use, Visionary presents all experiment set-up and device controls in one colour coded work-flow, under a single graphical user interface window.Key features of Visionary include:
|Confocality||0.6 μm (FWHM) with 1.4 N.A oil objective|
|Disc||1 sector structured illumination disc.|
|Minimum exposure||20 ms|
|Maximum frame rate||Up to 40 fps (1 colour)|
Up to 13 fps (3-colours)
|Imaging channels||3 integrated filter channels, DAPI, FITC, TRITC.|
|Detector||Integrated sCMOS 2x (2048 x 2048), 6.5 µm pixels.|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The height of versatility and adaptability, Clarity is extremely compact, having dimensions 390 x 220 x 135 mm (WDH) and weighing <6Kg. It can be mounted on upright and inverted microscopes, left or right ports and even on a macroscope.
Clarity’s unique combination of extended spectral range (370-750 nm) and high speed imaging with low photo-bleaching and low photo-toxicity makes it suitable for fixed and live specimens and allows its application to a wide range of research, including: