Organotypic brain slice culture sample, stained to detect axons (neurofilaments detected with alexa-595) and myelin (myelin basic protein detected with alexa-488).
Tilescan image collected with Aurox Clarity, fitted to a Nikon TiE with a 60x 1.4 NA Oil immersion objective, Lumencor Sola (kindly provided by Peter Call from AHF.de) and Hamamatsu Orca Flash 4.0.
10 micron thick stack, 20 slices, 24 tiles, collected in just over 15 minutes.
Courtesy of Dr Daniel Fulton from the Institute of Inflammation and Ageing at the University of Birmingham.