Tile of 3x1 images each a max projection image of 70 frame Z-stack over a 210µm range. Unity was fitted with a 10x 0.45NA lens. Green is myoII staining.
3x 70 images of 40 msec exposure (FITC) and 100ms (DsRED) per image. Time of acquisition was 13.3 secs per tile giving a total time for acquistion of 40 secs. Each tile was 1200 x 1200 µm. DAPI, FITC and DsRed LED Tri-band filter cube used.
Samples courtesy of Dr Florencia Cavodeass, St. George's University of London.
Fullsize image: 953 KB