Structured illumination spinning disk microscopy


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Light Sources

CoolLED pE-300Ultra

CoolLED's pE-300Ultra offers intense, broad-spectrum LED illumination for imaging the most common fluorescent stains with microsecond switching via multiple TTL inputs and the ability to mount inline excitation filters.

Pairing with high performance multi band filter sets enables imaging traditionally requiring a white light source and filter wheel.


  • Clean bright cross spectrum illumination
  • Specify for existing single and multi-band filter sets
  • Instant on/off
  • Simple installation and use
  • Stable and repeatable
  • Adapters to fit most microscopes
  • Precise intensity control in 1% steps (0-100%)
  • Excellent uniformity over field of view
  • Lamp life expected >25000 hours of operating time
  • Removable inline excitation filter holders
  • Individual microsecond channel triggering via TTL
  • Sequenced excitation from a single TTL-out with Sequence Runner

CoolLED pE-4000

The CoolLED pE-4000 is a broad spectrum (365-770 nm) illumination system for fluorescence microscopy with 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope.

For consistency and ease of use the system can be operated as a ‘White’ illumination system using simple on/off and intensity buttons, or in “Advanced” mode for total control of the excitation source for improved imaging and for selection and control of individual wavelength settings.


  • Broad LED spectrum: 365 – 770 nm
  • Four channel wavelength-grouping design
  • Individual control of selected LED wavelengths
  • Instant on/off - no shutters, no warm up or cool down
  • Precise intensity control in 1% steps (0-100%)
  • Excellent uniformity over field of view
  • Lamp life expected >25000 hours of operating time

Excelitas X-Cite 120PC Q

The Excelitas X-Cite 120PC Q is a computer controlled fluorescence microscope light source offering rich spectral sample excitation and a uniformly illuminated field of view from a 120W lamp which has been pre-aligned for convenience and has a guaranteed 2000 hours lifetime.

The X-cite 120PC Q also features software control and a standard adjustable iris, which may help minimise photo-bleaching.

Technical Specifications:
Illumination system includesLamp module, liquid light guide, microscope adaptor, software CD, grounded and shielded power cord, 9-pin serial cable, manual and quick start guide
LampPre-aligned proprietary 120W Mercury Vapor Short Arc
Lamp Life (hrs)2000 guaranteed
Software interfaceYes
Adjustable IrisYes
Foot pedal operationYes
Warm-up period (s)90 (typical)
Dimensions (mm)139 x 165 x 342
Weight (kg)3.18
AdaptersCompatible with wide-field fluorescence microscopes from all major manufacturers


Hamamatsu ORCA-Flash4.0 V3

The Hamamatsu ORCA-Flash4.0 V3 expertly handles applications ranging from the acquisition of beautiful scientific images to experiments that demand detection, quantification, and speed.

Improved on-board FPGA processing enables intelligent data reduction and highly refined in-camera, pixel-level calibrations. In combination with increased USB 3.0 frame rates, innovative triggering capabilities, a patented light sheet read-out mode, and individual camera noise characterization, the Hamamatsu ORCA-Flash4.0 V3 is the precision instrument for imaging.

Key specifications:
Read-out noise (e- med)1 (Standard scan 100 fps typical)
0.8 (Slow scan at 30 fps typical)
Resolution (pixels)2048 x 2048
Pixel size (μm)6.5
Dynamic range37000 : 1 (typical)
Quantum efficiency82% (peak QE at 560 nm)
InterfaceCamera Link / USB 3.0
Cooling methodPeltier cooling

PCO Edge 5.5 USB3 sCMOS

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

Photometrics Prime 95B

95% QE Backside Illuminated Scientific CMOS
Developed with award-winning technology, the Prime 95B Scientific CMOS camera allows you to overcome many difficult imaging challenges by converting nearly every available photon to useful signal through an incredible 95% Quantum Efficiency – by using the first available scientific CMOS sensor with backside illumination.

And now, the Prime 95B is available with advanced real-time processing features:
  • PrimeEnhance™ quantitatively increases the Signal to Noise Ratio by 3x-5x, increasing the clarity and quality of images

  • PrimeLocate™ dynamically evaluates acquired images and reduces the surplus of data generated during high speed super-resolution imaging.

Third Party Software

Oxford Instruments IMARIS

IMARIS is an easy to use 3D/4D image analysis software which seamlessly reconstructs and renders large 3D/4D datasets. At your disposal is a fully integrated platform to organize, visualise, analyse, and explore your images and present them in the best possible manner.

The big data capable Imaris Stitcher easily aligns and stitches image tiles and is able to export stitched images of terabytes in size.

Imaris ClearView-GPU™ deconvolution has been engineered to ensure the highest speeds and functionality with the largest possible datasets. Imaris ClearView-GPU™ deconvolution increases the resolution in all three dimensions: features that were previously indistinguishable become clearly separated.

Fiji / ImageJ

A third party software package, Fiji is a distribution of the widely used open source image processing software, ImageJ.

Fiji comes complete with a wide range of plugins used for the scientific analysis of multi-dimensional image data resulting from the life sciences.

Techniques supported:

  • Image processing
  • Colocalisation
  • Deconvolution
  • Registration
  • Segmentation
  • Tracking
  • Visualisation

Fiji is actively maintained, with regular update releases.

Media Cybernetics Image-Pro Premier 3D

Image-Pro Premier 3D is a commercial 3D image analysis software package from Media Cybernetics.

Designed for research professionals who need to view and measure structures in a range of spatial or temporal image dimensions, Image-Pro Premier 3D provides all the essential 3D analysis tools in one software package.

Key features include:

  • Automatically measure and count objects
  • Identify what to measure
  • Guided segmentation for measurement of complex 3D objects
  • Manual 3D measurements
  • 3D/4D Data Management
  • Automatically collect and analyse data from multiple experiments
  • 3D/4D high resolution image visualisation
  • 3D Clipping planes
  • 3D processing and dataset correction
  • Key frame animation movie publication

DRVision Aivia

Aivia represents the future of Microscopy Image Analysis. Powered by over 20 years of in-house R&D, Aivia is the most innovative and complete 2-to-5D image visualization, analysis and interpretation platform today.

Using state-of-the-art algorithm and software architecture, Aivia delivers top performance on critical tasks such as display of super-large images and analysis of complex biological phenomena.

Applications include:
  • Cell analysis
  • Relationship exploration
  • Object tracking
  • Neuron reconstruction
  • Colocalization
  • Object and pixel classification
  • Train and apply your own machine and deep learning models
Aivia is powered by a range of machine and deep learning technologies for image restoration, super resolution, segmentation, object classification and novelty detection.

Aivia is deployed as AiviaWeb (leveraging cloud computing and accessible via any browser) and/or as a desktop application. With Aivia, you are the analysis expert.

SVI Huygens

The Huygens Software includes many image processing options for brightfield and all types of fluorescence Microscope images. With Huygens it is possible to perform image deconvolution and restoration interactive analysis and volume visualization in 2D-4D multi-channel and time.

Additional Server and GPU-based Options ensure maximisation of hardware performance.

The Huygens software comes in different flavors offering solutions for both single users and multi-user facilities.

Huygens is also available as a web-based interface allowing submission of deconvolution tasks via your internet browser to the compute engine Huygens Core.


Microvolution software delivers nearly instantaneous deconvolution by combining intelligent software programming with the power of a GPU developed by Stanford scientists.

The software deconvolves images from widefield confocal two photon light sheet and HCA microscopes.

Blind deconvolution option improves noisy data such as deep tissue imaging. Multi-GPU options enable even giant images to be processed in seconds.

Micovolution allows you to:
  • Work more effectively in dim light and realize greater success rates with live-cell and time-lapse experiments;
  • Create cleaner measurements after deconvolution (e.g. colocalization FRET data neurite lengths fluorescence intensities);
  • Make adjustments to your microscopy experiments on the fly and collect more data from the same sample;



The extremely compact NUC, running Windows 10™ is the Aurox preferred PC for instrument control and image acquisition when combined with the Aurox Clarity laser free confocal device.

The NUC comes preloaded with Aurox Visionary software.

Typical specification:
  • 250GB SSD
  • 16GB RAM
  • Windows 10™ Home 64 Bit
  • Orico 7 Port USB 3 Hub

Windows 10 Shuttle PC

The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.

With this in mind, Aurox have configured a PC workstation specifically for use with Aurox Clarity confocal microscopy systems.

This PC workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.

  • High end Windows 10 Shuttle computer
  • Acquisition bandwidth for USB3 sCMOS camera support
  • SSD supports real time data acquisition
  • Multi-core processors for multi-threaded software
  • High performance graphics card with OpenGL
  • Real-time volume rendering


Aurox Ltd
Culham Science Centre
Oxfordshire. OX14 3DB.
Tel: +44 (0)1865 407814