Confocal Application Gallery

Rhizopus Sporangia

Maximum projection image recorded with 10x 0.45 NA objective.

Penicillium

Maximum projection image recorded with 10x 0.45 NA objective.

Cup Fungus

Clarity LFC on Nikon TiE with Orca Flash 4.0 and CoolLED pe300. 10x 0.45 dry objective.

Acryria Slime Mould tilescan

Arcyria Slime Mould red autofluorescence 4 x 3 tilescan recorded using Unity. 109 slices. 3 micron steps. Recorded using 10x, 0.45 NA dry objective.

Dictydium Slime Mould tilescan

Tilescan image of Dictydium slime mould recorded on Unity using a 10x, 0.45 NA dry objective. 4 x 2 tilescan red autofluorescence. 111 slices, 3 micron step size. All images have been cropped from original image size and stitched using Andor Imaris. Video produced in Imaris

Slime mould samples from Carolina Biological Supply, supplied by Blades Biological

Fluocells tilescan

Tilescan of Invitrogen Fluocells prepared slide #1: BPAE cells with Mitotracker Red CMXRos, AlexaFluor 488 Phalloidin and DAPI.60x 0.4NA Oil immersion lens. 12 tiles, 53 Z slices, recorded in 3 minutes.

Brassaca

Images recorded on a Nikon Ti-E using a 63x 1.4 N.A Oil objective with GFP and DsRED filter cubes.

Shepherds Purse 1

Maximum projection image recorded with 10x 0.45 NA objective.

Shepherds Purse 2

Maximum projection image recorded with 10x 0.45 NA objective.

Root tip

Maximum projection image recorded with 10x 0.45 NA objective.

Root hairs

Maximum projection image recorded with 10x 0.45 NA objective.

Privet leaf

Maximum projection image recorded with 10x 0.45 NA objective.

Lily flower bud

Maximum projection image recorded with 10x 0.45 NA objective.

Corn stem

Maximum projection image recorded with 10x 0.45 NA objective.

Corn leaf

Maximum projection image recorded with 10x 0.45 NA objective.

Buttercup root

Maximum projection image recorded with 10x 0.45 NA objective.

Bass wood stem

Maximum projection image recorded with 10x 0.45 NA objective.

Pine Pollen

3D movie of a pine pollen

Pollen grain

Pollen grain autofluorescence. Clarity LFC on Nikon TiE with Orca Flash 4.0 and CoolLED pe300. 10x 0.45 dry objective.

Zebrafish tilescan

Tile of 3x1 images each a Z-projection image of 70 frame Z-stack over a 210µm range. Unity was fitted with a 10x 0.45NA lens. Green is myoII staining.

3x 70 images of 40 msec exposure (FITC) and 100ms (DsRED) per image. Time of acquisition was 13.3 secs per tile giving a total time for acquistion of 40 secs. Each tile was 1200 x 1200 µm. DAPI, FITC and DsRed LED Tri-band filter cube used.

Samples courtesy of Dr Florencia Cavodeass, St. George's University of London.

Head detail of C.elegans

Maximum projection 3D movie of a C.elegans head.

Samples courtesy of Dr Huajiang Xiong & Prof Alison Woollard, Department of Biochemistry, University of Oxford.

Single C.elegans

Maximum projection image of a single C.elegans worm, shown head to tail.

Samples courtesy of Dr Huajiang Xiong & Prof Alison Woollard, Department of Biochemistry, University of Oxford.

C.elegans group

Maximum projection image of C.elegans. The image shows three C.elegans ranging from an egg (top right) through a juvenile (top) to an adult (bottom).

Total exposure time = 13.8 sec.

GFP filter cube, 40x 0.95 NA objective, z-stack = 22 slices of 0.6 μm.

Samples courtesy of Dr Huajiang Xiong & Prof Alison Woollard, Department of Biochemistry, University of Oxford.

C.elegans

3D movie of body tissue muscle wall cells and Vulva muscle cells.

Total exposure time = 14 sec.

GFP filter cube, 40x 0.95 NA objective, z-stack = 22 slices of 0.6 μm.

Samples courtesy of Dr Huajiang Xiong & Prof Alison Woollard, Department of Biochemistry, University of Oxford.

Zebrafish Heart

Fast timelapse series of a beating zebra fish embryo heart acquired with Andor DSD2. Imaging switches from confocal to widefield halfway through the series.

Movie courtesy of Didier Hentsch (IGBMC, Strasbourg, France) and Bruno Combettes (Andor Technology, UK).

Organotypic brain slice culture

Organotypic brain slice culture sample, stained to detect axons (neurofilaments detected with alexa-595) and myelin (myelin basic protein detected with alexa-488).

Tilescan image collected with Aurox Clarity, fitted to a Nikon TiE with a 60x 1.4 NA Oil immersion objective, Lumencor Sola (kindly provided by Peter Call from AHF.de) and Hamamatsu Orca Flash 4.0.

10 micron thick stack, 20 slices, 24 tiles, collected in just over 15 minutes.

Courtesy of Dr Daniel Fulton from the Institute of Inflammation and Ageing at the University of Birmingham.

Mouse whole mount brain

Mouse whole mount brain. WGA-Alexa594 (blood vessels and capillaries). Supplemented with Alex488-labeled polystrene beads. 19 z-slices each 8 um apart (total stack thickness 152 um). Deconvoluted with Microvolution plug-in for FIJI MAX projection.

Imaged with: Clarity LFC on a Leica M205FCA stereo microscope with 2X macro objective, CoolLED pe300Ultra via LLQ and Hamamatsu ORCA Flash 4.

Sample courtesy of Dr. Mika Kaakinen, University of Oulu, Finland.

Mosquito heads (male and female comparison) tilescan

Tile scan Z-projection image of male and female autofluorescence mosquito heads.

Imaged with Aurox Unity and 10 x 0.45 NA, dry objective.

DAPI (80 ms exposure) FITC (80 ms exposure, but not shown in final image) /DsRED (300 ms exposure) excitation channels used for imaging.

94 slices, 3 micron steps, 12 tiles.

Total time of acquisition = 147 seconds.

Prepared Slide from Brunel Microscopes Ltd

Red flour weevil underbelly tilescan (autofluorescence)

Tile scan Z-projection image of autofluorescent Red flour weevil underbelly.

Imaged with Aurox Unity and 10 x 0.45 NA, dry objective.

DAPI (80 ms exposure) FITC (20 ms exposure) /DsRED (40 ms exposure) excitation channels used for imaging.

133 slices, 3 micron steps, 12 tiles.

Total time of acquisition = 87 seconds.

Prepared Slide from Brunel Microscopes Ltd

Flea tilescan

3 x 3 tilescan autofluorescence image of a Flea whole mount (Brunel Microscopes Ltd). Recorded on Unity. Tilescans stitched and video created using Andor Imaris. 101 slices, DsRED filter set.