Embryonic stem cell lines are now widely established in the research community using traditional in vitro culture.
The cell lines remain undifferentiated with normal nuclei during subculture, but are capable of differentiation into virtually any type of tissue.
They will first become stem cells according to the specific culture conditions before differentiation into e.g. neurons, muscle cells, vascular endothelial cells and blood cells.
The Olympus IX83 is a fully motorised inverted microscope which can be used in Fluorescence, Differential Interference Contrast (DIC), Phase Contrast, Simple Polarised Light, Brightfield and Relief Contrast observation modes.
The IX83 is available in one-deck or two-deck configurations making it suitable for a range of imaging techniques from casual documentation to more demanding techniques such as long-term time lapse imaging
The IX83's open frame provides ease of access to the light path and enables the ready addition and or exchange of a range of deck modules for extended functionality.
The IX3-ZDC2 Z drift compensator module can be easily added to the microscope to maintain continuous focus throughout extended time-lapse experiments and this together with the microscope's large field number (FN22, left side port) provide an ideal platform for live cell imaging.
The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The Zyla 5.5 USB3 is the latest 16 bit sCMOS based camera from Andor. It offers high speed, high sensitivity imaging from a compact device that is ideal for low light microscopy.
|Thermal stabilization (°C )||0|
|Read-out noise (e- rms)||1.2|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||25000 : 1|
|Quantum efficiency||Up to 60%|
|Maximum frame rate (fps)||40|
|Read-out modes||Rolling and global shutter|
|Dimensions (mm)||133 x 82 x 80|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.
Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
|Type||Super Plan Apochromat|
|Working Distance (mm)||0.6|
The Excilitas X-Cite 120PC Q is a computer controlled fluorescence microscope light source offering rich spectral sample excitation and a uniformly illuminated field of view from a 120W lamp which has been pre-aligned for convenience and has a guaranteed 2000 hours lifetime.
The X-cite 120PC Q also features software control and a standard adjustable iris, which may help minimise photo-bleaching.
|Illumination system includes||Lamp module, liquid light guide, microscope adaptor, software CD, grounded and shielded power cord, 9-pin serial cable, manual and quick start guide|
|Lamp||Pre-aligned proprietary 120W Mercury Vapor Short Arc|
|Lamp Life (hrs)||2000 guaranteed|
|Foot pedal operation||Yes|
|Warm-up period (s)||90 (typical)|
|Dimensions (mm)||139 x 165 x 342|
|Adapters||Compatible with wide-field fluorescence microscopes from all major manufacturers|
Constructed from polycarbonate panels, the OkoLab cage incubator creates a large temperature controlled volume around the microscope stage, nosepiece and objectives. The incubator is supported by posts resting on the vibration isolation table and the enclosure closely follows the profile of the microscope with all electronics located outside the enclosure. It's flat panel construction makes for easy assembly and disassembly and simplifies modifications to single panel changes, whilst maintaining focus stability.
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: