Fluorescent protein dynamics (e.g. translocation)

Imaging of fluorescent proteins using confocal microscopy can uniquely provide detailed cellular structure and function through the use of fluorescent proteins as tracers of intercellular protein trafficking and the localisation and dynamics of proteins and organelles.

The selection of appropriate objectives, emission filters and detector are critical to these confocal microscopy studies.

aurox confocal microscope title

The following is a suggested Clarity system configuration for this application:

The Olympus BX is a fully motorised upright microscope which can be used in Fluorescence, Differential Interference Contrast (DIC), Phase Contrast, Simple Polarised Light, Brightfield and Darkfield observation modes.

The BX range is unusually focused by driving the motorised nosepiece in the z-direction. This means that the microscope stage remains fixed and affords greater stability.

The BX stage can be driven using its ultrasonic Piezo motor technology, which provide silent, smooth and precise operation, or it can be positioned manually; allowing for fast gross sample alignment. In motorised or manual operation the BX's high accuracy encoders provide continuous read-out of the X and Y position of the stage.

Key features:

  • Fully motorised
  • Motorised focus using the nosepiece z-direction
  • Motorised objective change
  • Touch panel display / control
  • Computer controllable

aurox confocal microscope Olympus BX - upright

The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.

Key specifications:
Thermal stabilization (°C )0 / +5
Read-out noise (e- med)1.0
Resolution (pixels)2560 x 2160 (5.5 Megapixel)
Dynamic range30000 : 1
Quantum efficiency>60%
Exposure time (secs)0.1 to 2
Maximum frame rate (fps)30
Read-out modesRolling or global / global reset functionality
Lightsheet modeYes
InterfaceUSB 3.0
SensorBlack and white sCMOS
Dimensions (mm)102 x 79 x 70

aurox confocal microscope PCO Edge 5.5 USB3 sCMOS

The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.

Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.

The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.

Key features of the software:

  • Instrument set-up and calibration
  • Experiment set-up and control
  • Real time confocal image quality inspection tool
  • Supports a range of digital camera manufacturer sCMOS devices, including, PCO and Andor
  • Outputs data in OME Tiff format

aurox confocal microscope Aurox Visionary

TypeSuper Plan Apochromat
Numerical Aperture0.95
Working Distance (mm)0.18

aurox confocal microscope Olympus UPLSAPO 40x2

The Cool LED pE-4000 is a broad spectrum (365-770 nm) illumination system for fluorescence microscopy with 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope.

For consistency and ease of use the system can be operated as a ‘White’ illumination system using simple on/off and intensity buttons, or in “Advanced” mode for total control of the excitation source for improved imaging and for selection and control of individual wavelength settings.


  • Broad LED spectrum: 365 – 770 nm
  • Four channel wavelength-grouping design
  • Individual control of selected LED wavelengths
  • Instant on/off - no shutters, no warm up or cool down
  • Precise intensity control in 1% steps (0-100%)
  • Excellent uniformity over field of view
  • Lamp life expected >25000 hours of operating time

aurox confocal microscope Cool LED pE-4000

Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.

Service contracts can be purchased in 1 year or up to 5 year intervals.

Aurox service contracts include:

  • 1x Annual preventative maintenance service visit
  • Replacement parts and labour (excludes any consumables)
  • Software updates