Immunofluorescence is used in almost all aspects of biology and is widely used in research and clinical diagnostics applications, including the evaluation of cells in suspension, cultured cells, tissue, beads and microarrays for the detection of specific proteins.
In Immunofluorescence, antibodies are chemically labelled using fluorescent dyes and these antibodies then bind, either directly or indirectly to the antigen of interest.
The antigen can then be detected using fluorescence and visualised using confocal microscopy.
The Nikon Eclipse Ti-E inverted microscope has been designed for the most advanced live cell imaging applications including TIRF, confocal imaging, FRET, photo activation and microinjection.
The high speed motorised nosepiece, fluorescence filters and microscope stage allow for high throughput screening during multi-dimensional experiments, whilst the automatic focus correction system continuously corrects for any drift during long duration time-lapse imaging.
The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The PCO Edge 5.5 is an extremely compact 16 bit camera which is equipped with a scientific CMOS sensor and is ideally suited for measuring images at high resolution and high frame rates.
|Thermal stabilization (°C )||0 / +5|
|Read-out noise (e- med)||1.0|
|Resolution (pixels)||2560 x 2160 (5.5 Megapixel)|
|Dynamic range||30000 : 1|
|Exposure time (secs)||0.1 to 2|
|Maximum frame rate (fps)||30|
|Read-out modes||Rolling or global / global reset functionality|
|Sensor||Black and white sCMOS|
|Dimensions (mm)||102 x 79 x 70|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
|Working Distance (mm)||0.13|
The Cool LED pE-4000 is a broad spectrum (365-770 nm) illumination system for fluorescence microscopy with 16 selectable LED wavelengths that can be matched to the filters and fluorophores of almost any microscope.
For consistency and ease of use the system can be operated as a ‘White’ illumination system using simple on/off and intensity buttons, or in “Advanced” mode for total control of the excitation source for improved imaging and for selection and control of individual wavelength settings.
Aurox offer service and support contracts to cover all Aurox Clarity confocal microscopy systems.
Service contracts can be purchased in 1 year or up to 5 year intervals.
Aurox service contracts include: