The easy and affordable upgrade
Introducing the Aurox Clarity, the compact, easy and affordable upgrade to laser-free confocal imaging using your existing fluorescence microscope.
Clarity packs 15 years of Aurox confocal microscopy and optical technology into a compact, high performance confocal imaging system.
Aurox’s patented structured illumination
Designed to fit virtually any microscope, Clarity uses Aurox’s patented structured illumination technology and a spinning disc to achieve high resolution, high quality confocal images, fast, easily and affordably.
Clarity has a standard microscope C-mount connector and a range of coupling adaptors / spacers to fit historical and new microscope models from all major manufacturers.
The height of versatility and adaptability, Clarity is extremely compact, having dimensions 390 x 220 x 135 mm (WDH) and weighing <6Kg. It can be mounted on upright and inverted microscopes, left or right camera ports.
Microscopes and Z drives
- Nikon TE2000
- Nikon TiE
- Nikon Ti2
- Leica DMI
- Leica DM6
- Leica M Stereo
- Olympus IX81/BX61
- Olympus IX83
- Zeiss AxioObserver
- Zeiss AxioVert
- Zeiss AxioSkop
- Ludl MAC (Z drive)
- Prior Z stage
- Thorlabs MFC1 (z-drive)
- PI P-721 (piezo z-drive)
Connect to CMOS and CCD
Clarity can be connected to a wide range of large format sCMOS and CCD cameras. Selected cameras can be purchased directly from Aurox with your Clarity.
If you already have a camera, a wide range are supported by our Visionary software for use with Clarity.
Supported cameras/detectors include:
- Hamamatsu Orca Flash 4.0 V3
- Hamamatsu Orca Flash 4 LT
- Hamamatsu Orca Flash 4 V2 (USB 3.0)
- PCO Panda
- PCO Edge 5.5 USB3
- Photometrics 95 Prime BSI
- Photometrics 95 Prime B
- Andor Zyla 5.5 USB
- Tucsan Dhyana BSI
Internal 4-position filter cube turret
Clarity incorporates an internal 4-position filter cube turret, accessed via an external door. The turret accepts Aurox’s custom sized filter cubes which incorporate both emission and excitation filters. These filter cubes are user exchangeable and quick and easy to replace by hand and without tools.
A wide range of Aurox filter cubes are available to cover the commonly used bandwidths, which include multi-pass quad band filter cubes.
Custom filter cubes can be produced for your specific research application needs.
Fast and easy to use
Clarity comes with the Aurox Visionary device control and image acquisition software.
Fast and easy to use, Visionary presents all experiment set-up and device controls in one colour coded work-flow, under a single graphical user interface window.
Key features of Visionary include:
- z-stack imaging
- Mosaic image tiling
- Time-lapse & multi-channel multi-position imaging
- Confocal, wide-field & bright-field modes
- 3 sectioning modes for optimised resolution or speed
- OME-TIFF file format
- One-click image export
For more complicated experimental set-up or control, Clarity can also be controlled using a customised Aurox Micromanager.
Up to 100 frames per second
The high speed version, ClarityHS provides up to 100 frames per second imaging when paired with a compatible high speed camera e.g. Hamamatsu Flash 4.0 V3 with “Camera link option” and a Shuttle computer.
Stand alone instrument and control
Clarity has the option of delivery with an instrument control computer installed with the Aurox Visionary software. The computer is connected by USB and can be either a compact NUC or a Shuttle PC.
LED and metal halide light sources
Designed for use with LED and metal halide light sources, Clarity is free from laser safety restrictions and benefits from low photo-toxicity, low-bleaching, low maintenance and low cost of ownership.
Selected light sources are available directly from Aurox with your Clarity.
If you already have a light source then please contact us to discover whether or not it is supported.
Light source options include:
- Andor AMH200
- Exfo X-Cite 120PC-Q
- Exfo X-Cite 120 LED
- Lumencor Sola
- Lumencor Spectra X
- Cool LED pE-300
- Cool LED pE-400
- Cool LED pE- 800
- Cool LED pE-4000
- Prior Lumen 200
| Key specifications | Clarity |
|---|---|
| Operating principle | Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample |
| Confocality (PSF) | 3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens |
| Widefield By-pass | Selectable mode completely removes disk from the imaging path |
| Minimum exposure | 20 ms or 10 ms (HS) |
| Max frame rates | 50 fps - 100 fps (HS) |
| Imaging channels | 4 user-exchangeable cubes on an internal turret |
| Channel switching time | <200 ms |
| Excitation range | 370 - 700 nm |
| Emission range | 410 - 750 nm |