Virtually any laboratory microscope has the potential to be upgraded to become a confocal microscope with the addition of the Aurox Clarity Laser Free Confocal (LFC). The Aurox Clarity LFC is a laser free confocal device delivering extraordinary imaging performance. It's compact patented optical design and laser-free operation, provide for ease of retrofit to an existing fluorescence microscope, or addition to most new models currently available. The benefits are an affordable confocal device for your own laboratory, eliminating the need to use core facilities.
|Operating principle||Structured illumination, spinning disk. 3 grid densities for matching sectioning and throughput to objective and sample|
|Confocality (PSF)||3 sectors provide adjustable sectioning strength down to 0.6 μm (FWHM) with 1.4 NA oil immersion lens|
|Widefield By-pass||Selectable mode completely removes disk from the imaging path|
|Disk rotation speed||3000 rpm|
|Minimum exposure||20 ms (1 full disk rotation)|
|Frame rates (max)||22 fps (12-bit confocal imaging, full 2.3 Mpix frame size, no binning), up to 50 fps with 2x2 binning|
|Spectral channels||4 user-replaceable cubes on a fast internal filter turret|
|Filter switching time||<200 ms|
|Excitation range||370 - 700 nm|
|Emission range||410 - 750 nm|
The Aurox Visionary software has been specially developed for the acquisition of live cell imaging data using the Aurox Clarity LFC unit as part of a laser free confocal microscopy system.
Designed with simplicity and ease of use at its core, the software provides a single graphical user interface, which relates device and experimental settings directly to the connected instrument hardware.
The Visionary software provides for the optimisation and control of the instrument set-up and calibration followed by the set-up and image acquisition control of the confocal microscopy experiment.
Key features of the software:
The handling, processing and visualisation of multi-dimensional confocal microscopy live cell images are extremely computer intensive and require the use of a high performance desktop computer.
With this in mind, Aurox have configured a PC Workstation specifically for use with Aurox Clarity confocal microscopy systems.
This PC Workstation has been optimised for handling large experimental data sets and for use with the Aurox Visionary software.
Imaris is a commercial software package from Bitplane, designed for the data visualisation, analysis, segmentation and interpretation of 3D/4D microscopy datasets.
Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with three- and four-dimensional multi-channel images of any size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.
A third party software package, Fiji is a distribution of the widely used open source image processing software, ImageJ.
Fiji comes complete with a wide range of plugins used for the scientific analysis of multi-dimensional image data resulting from the life sciences.
Fiji is actively maintained, with regular update releases.
More information is available from www.fiji.sc
Image-Pro Premier 3D is a commercial 3D image analysis software package from Media Cybernetics.
Designed for research professionals who need to view and measure structures in a range of spatial or temporal image dimensions, Image-Pro Premier 3D provides all the essential 3D analysis tools in one software package.
Key features include: